Diversidade de cepas de Stenotrophomonas maltophilia isoladas entre 1958 e 2021 e genotipadas por multilocus sequence typing
Palavras-chave:
Epidemiologia, MLST, Caracterização molecular, Stenotrophomonas maltophilia
Resumo
O objetivo deste estudo foi avaliar os dados in silico de cepas de S. maltophilia (n=974) caracterizadas por Multilocus Sequence Typing (MLST) disponíveis no banco PubMLST isoladas entre 1958 e 2020. As cepas com perfil alélico completo (n=972) foram analisadas utilizando a árvore filogenética neighbor-joining, a ferramenta minimum spanning tree e o algoritmo eBURST. O índice de Simpson foi aplicado para calcular o poder de resolução do MLST para tipificação. A maioria das cepas depositadas são de origem clínica (87,6%) e oriundas da Ásia (47,1%). As cepas de S. maltophilia apresentaram alta diversidade genética quando avaliadas, sendo identificadas em 679 ST distintos, uma taxa de ~1,4 cepa/ST. O índice de Simpson calculado foi de 0,99. O Brasil possui 10 cepas depositadas classificadas em sete ST distintos, sendo quatro isolados de infecções em humanos. Em conclusão, o MLST apresentou-se como uma ferramenta poderosa para investigações epidemiológicas de cepas de S. maltophilia.Referências
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AGÊNCIA NACIONAL DE VIGILÂNCIA SANITÁRIA (Anvisa). Gerência de Investigações e Prevenção das Infecções e dos Eventos Adversos (Gipea)/Gerência Geral de Tecnologia em Serviços de Saúde (GGTES). Investigação e Controle de Bactérias Multirresistentes, p. 21, 2017.
ALIKHAN, N. F.; ZHEMIN, Z.; SERGEANT, M. J.; ACHTMAN, M. A genomic overview of the population structure of Salmonella. PLoS Genetics, v. 14, n. 4, p. e1007261, 2018.
ANDRADE, L. O. Métodos rápidos para identificação microbiana aplicados ao monitoramento ambiental de salas limpas: ênfase na tecnologia MALDI-TOF. 2017. 143 f. Dissertação (Mestrado em Fármaco e Medicamentos) – Universidade de São Paulo, São Paulo, 2017.
BAIOU, A. et al. Clinical characteristics and risk factors for the isolation of multi-drug-resistant Gram-negative bacteria from critically ill patients with COVID-19. The Journal of Hospital Infection, v. 110, p 165-171, 2021.
BIOMÉRIEUX. Disponível em: . Acesso em: 30 de jun. 2021.
BRAGA, F. S. et al. Genomic information on Stenotrophomonas maltophilia ST264 isolated from a cystic fibrosis pediatric patient in Brazil. Braz J Microbiol, v. 51, n. 3, p. 1125-1127, 2020.
COSTA, L. V. Avaliação da diversidade microbiana presente em sistemas gerador de água purificada dedicada à produção de peniceilínicos em Farmanguinhos. Fiocruz, 2014. 88f. Dissertação (Mestrado em Gestão, Pesquisa e Desenvolvimento na Indústria Farmacêutica), Rio de Janeiro, 2014.
COSTA, P. V. et al. Diversity of Cronobacter genus isolated between 1970 and 2019 on the American continent and genotyped using multi-locus sequence typing. FEMS Microbiol Lett, v. 368, n. 5, 2021.
CHANG, Y. T. et al. Update on infections caused by Stenotrophomonas maltophilia with particular attention to resistance mechanisms and therapeutic options. Front Microbiol, v. 6, n. 893, 2015.
CHONG, W. H. et al. State-of-the-art review of secondary pulmonary infections in patients with COVID-19 pneumonia. Infection, v. 11, p. 1–15. 11, 2021.
CROXATTO, A.; PROD'HOM, G.; GREUB, G. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiology Reviews, v. 36, n. 2, p. 380-407, 2012.
FEIL, E. et al. eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol, v. 86, p. 1518-1530, 2004.
GAUTAM, V. et al. MALDI-TOF mass spectrometry: An emerging tool for unequivocal identification of non-fermenting Gram-negative bacilli. The Indian journal of medical research, v. 145, n. 5, p. 665–672, 2017.
HUNTER, P.; GASTON, M. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol, v. 26, p. 2465–2466, 1988.
KAISER, S.; BIEHLER, K.; JONAS, D. A Stenotrophomonas maltophilia multilocus sequence typing scheme for inferring population structure. J Bacteriol, v. 191, n. 9, p. 2934-2943, 2009.
LAGE, R. V. et al. Phenotypic characterization of Stenotrophomonas maltophilia as a tracking tool for investigation in a pharmaceutical industry. Resumo (Anais). II Congresso Online Nacional de Ciências Farmacêuticas, 2021.
LANGFORD, B. J. et al. Bacterial co-infection and secondary infection in patients with COVID-19: a living rapid review and meta-analysis. Clin Microbiol Infect, v. 26, n. 12, p. 1622-1629, 2020.
LETUNIC, I.; BORK, P. Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res, v. 44, p. 242-245, 2016.
LOONEN, A. J. M. et al. Comparative Study Using Phenotypic, Genotypic, and Proteomics Methods for Identification of Coagulase-Negative Staphylococci. American Society for Microbiology. J Clin Microbiol, p. 1437–1439, 2012.
MIRZAEI, R. et al. Bacterial co-infections with SARS-CoV-2. IUBMB life, v.72, n. 10, p. 2097-2111, 2020.
MOJICA, M. F. et al. Population Structure, Molecular Epidemiology, and β-Lactamase Diversity among Stenotrophomonas maltophilia Isolates in the United States. mBio, v. 10, n. 4, 2019.
MONTOYA, E. H. et al. Discrimination of biofilm-producing Stenotrophomonas maltophilia clinical strains by matrix-assisted laser desorption ionization-time of flight. PLoS OnE, v. 15, n. 12, 2020.
PAGE, A. J. et al. Comparison of classical multi-locus sequence typing software for next generation sequencing data. Microb Genom, v. 3, n. 8, 2017.
PubMLST. Public databases for molecular typing and microbial genome diversity. Stenotrophomonas maltophilia. Stenotrophomonas maltophilia isolates. Isolate information. Full information on isolate SM79 (id:637). 2021a Disponível em https://pubmlst.org/bigsdb?page=info&db=pubmlst_smaltophilia_isolates&id=637. Acesso em: 30 de jun. 2021.
PubMLST. Public databases for molecular typing and microbial genome diversity. Stenotrophomonas maltophilia. Stenotrophomonas maltophilia isolates. Isolate information. Full information on isolate SM115 (id:638). 2021b. Disponível em https://pubmlst.org/bigsdb?page=info&db=pubmlst_smaltophilia_isolates&id=638. Acesso em: 30 de jun. 2021.
RAVE, A. F. G., et al. Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents. Braz J Biol, v. 79, n. 4, p. 555-565, 2019.
RIZEK, C. F. et al. Multidrug-resistant Stenotrophomonas maltophilia: Description of new MLST profiles and resistance and virulence genes using whole-genome sequencing. J Glob Antimicrob Resist, v. 15, p. 212-214, 2018.
SILVA, F. P. Análises genômicas de três isolados do gênero Stenotrophomonas oriundos de vermicomposto. Dissertação (Mestrado). Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, Rio de Janeiro, 2019.
SOSTARICH, A. M. et al. Impact of multiresistance of gram-negative bacteria in bloodstream infection on mortality rates and length of stay. Infection; v. 36, n.1, p. 31- 35, 2008.
TRIFONOVA, A.; STRATEVA, T. Stenotrophomonas maltophilia - a low-grade pathogen with numerous virulence factors. Infectious diseases, v. 51. n. 3, p. 168-178, 2019.
VASCONCELOS, L. et al. Phenotypic characterization of Pseudomonas aeruginosa as a tracking tool for investigation in a pharmaceutical industry. Resumo (Anais). V International Symposium on Immunobiologicals, p, 48, 2021.
WORLD HEALTH ORGANIZATION (WHO). Public Health Importance of Antimicrobial Resistance. Drug resistance. Disponível em:
ZHOU, Z. et al. GrapeTree: visualization of core genomic relationships among 100,000 bacterial pathogens. Genome Research, v. 28, p. 1395-1304, 2018.
AGÊNCIA NACIONAL DE VIGILÂNCIA SANITÁRIA (Anvisa). Gerência de Investigações e Prevenção das Infecções e dos Eventos Adversos (Gipea)/Gerência Geral de Tecnologia em Serviços de Saúde (GGTES). Investigação e Controle de Bactérias Multirresistentes, p. 21, 2017.
ALIKHAN, N. F.; ZHEMIN, Z.; SERGEANT, M. J.; ACHTMAN, M. A genomic overview of the population structure of Salmonella. PLoS Genetics, v. 14, n. 4, p. e1007261, 2018.
ANDRADE, L. O. Métodos rápidos para identificação microbiana aplicados ao monitoramento ambiental de salas limpas: ênfase na tecnologia MALDI-TOF. 2017. 143 f. Dissertação (Mestrado em Fármaco e Medicamentos) – Universidade de São Paulo, São Paulo, 2017.
BAIOU, A. et al. Clinical characteristics and risk factors for the isolation of multi-drug-resistant Gram-negative bacteria from critically ill patients with COVID-19. The Journal of Hospital Infection, v. 110, p 165-171, 2021.
BIOMÉRIEUX. Disponível em:
BRAGA, F. S. et al. Genomic information on Stenotrophomonas maltophilia ST264 isolated from a cystic fibrosis pediatric patient in Brazil. Braz J Microbiol, v. 51, n. 3, p. 1125-1127, 2020.
COSTA, L. V. Avaliação da diversidade microbiana presente em sistemas gerador de água purificada dedicada à produção de peniceilínicos em Farmanguinhos. Fiocruz, 2014. 88f. Dissertação (Mestrado em Gestão, Pesquisa e Desenvolvimento na Indústria Farmacêutica), Rio de Janeiro, 2014.
COSTA, P. V. et al. Diversity of Cronobacter genus isolated between 1970 and 2019 on the American continent and genotyped using multi-locus sequence typing. FEMS Microbiol Lett, v. 368, n. 5, 2021.
CHANG, Y. T. et al. Update on infections caused by Stenotrophomonas maltophilia with particular attention to resistance mechanisms and therapeutic options. Front Microbiol, v. 6, n. 893, 2015.
CHONG, W. H. et al. State-of-the-art review of secondary pulmonary infections in patients with COVID-19 pneumonia. Infection, v. 11, p. 1–15. 11, 2021.
CROXATTO, A.; PROD'HOM, G.; GREUB, G. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiology Reviews, v. 36, n. 2, p. 380-407, 2012.
FEIL, E. et al. eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol, v. 86, p. 1518-1530, 2004.
GAUTAM, V. et al. MALDI-TOF mass spectrometry: An emerging tool for unequivocal identification of non-fermenting Gram-negative bacilli. The Indian journal of medical research, v. 145, n. 5, p. 665–672, 2017.
HUNTER, P.; GASTON, M. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol, v. 26, p. 2465–2466, 1988.
KAISER, S.; BIEHLER, K.; JONAS, D. A Stenotrophomonas maltophilia multilocus sequence typing scheme for inferring population structure. J Bacteriol, v. 191, n. 9, p. 2934-2943, 2009.
LAGE, R. V. et al. Phenotypic characterization of Stenotrophomonas maltophilia as a tracking tool for investigation in a pharmaceutical industry. Resumo (Anais). II Congresso Online Nacional de Ciências Farmacêuticas, 2021.
LANGFORD, B. J. et al. Bacterial co-infection and secondary infection in patients with COVID-19: a living rapid review and meta-analysis. Clin Microbiol Infect, v. 26, n. 12, p. 1622-1629, 2020.
LETUNIC, I.; BORK, P. Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res, v. 44, p. 242-245, 2016.
LOONEN, A. J. M. et al. Comparative Study Using Phenotypic, Genotypic, and Proteomics Methods for Identification of Coagulase-Negative Staphylococci. American Society for Microbiology. J Clin Microbiol, p. 1437–1439, 2012.
MIRZAEI, R. et al. Bacterial co-infections with SARS-CoV-2. IUBMB life, v.72, n. 10, p. 2097-2111, 2020.
MOJICA, M. F. et al. Population Structure, Molecular Epidemiology, and β-Lactamase Diversity among Stenotrophomonas maltophilia Isolates in the United States. mBio, v. 10, n. 4, 2019.
MONTOYA, E. H. et al. Discrimination of biofilm-producing Stenotrophomonas maltophilia clinical strains by matrix-assisted laser desorption ionization-time of flight. PLoS OnE, v. 15, n. 12, 2020.
PAGE, A. J. et al. Comparison of classical multi-locus sequence typing software for next generation sequencing data. Microb Genom, v. 3, n. 8, 2017.
PubMLST. Public databases for molecular typing and microbial genome diversity. Stenotrophomonas maltophilia. Stenotrophomonas maltophilia isolates. Isolate information. Full information on isolate SM79 (id:637). 2021a Disponível em https://pubmlst.org/bigsdb?page=info&db=pubmlst_smaltophilia_isolates&id=637. Acesso em: 30 de jun. 2021.
PubMLST. Public databases for molecular typing and microbial genome diversity. Stenotrophomonas maltophilia. Stenotrophomonas maltophilia isolates. Isolate information. Full information on isolate SM115 (id:638). 2021b. Disponível em https://pubmlst.org/bigsdb?page=info&db=pubmlst_smaltophilia_isolates&id=638. Acesso em: 30 de jun. 2021.
RAVE, A. F. G., et al. Biochemical identification techniques and antibiotic susceptibility profile of lipolytic ambiental bacteria from effluents. Braz J Biol, v. 79, n. 4, p. 555-565, 2019.
RIZEK, C. F. et al. Multidrug-resistant Stenotrophomonas maltophilia: Description of new MLST profiles and resistance and virulence genes using whole-genome sequencing. J Glob Antimicrob Resist, v. 15, p. 212-214, 2018.
SILVA, F. P. Análises genômicas de três isolados do gênero Stenotrophomonas oriundos de vermicomposto. Dissertação (Mestrado). Universidade Estadual do Norte Fluminense (UENF), Campos dos Goytacazes, Rio de Janeiro, 2019.
SOSTARICH, A. M. et al. Impact of multiresistance of gram-negative bacteria in bloodstream infection on mortality rates and length of stay. Infection; v. 36, n.1, p. 31- 35, 2008.
TRIFONOVA, A.; STRATEVA, T. Stenotrophomonas maltophilia - a low-grade pathogen with numerous virulence factors. Infectious diseases, v. 51. n. 3, p. 168-178, 2019.
VASCONCELOS, L. et al. Phenotypic characterization of Pseudomonas aeruginosa as a tracking tool for investigation in a pharmaceutical industry. Resumo (Anais). V International Symposium on Immunobiologicals, p, 48, 2021.
WORLD HEALTH ORGANIZATION (WHO). Public Health Importance of Antimicrobial Resistance. Drug resistance. Disponível em:
ZHOU, Z. et al. GrapeTree: visualization of core genomic relationships among 100,000 bacterial pathogens. Genome Research, v. 28, p. 1395-1304, 2018.
Publicado
2021-07-06
Como Citar
LAGE, R. V.; COSTA, P.; DA COSTA, L.; VILLAS BÔAS, M. H.; BRANDÃO, M. L. Diversidade de cepas de Stenotrophomonas maltophilia isoladas entre 1958 e 2021 e genotipadas por multilocus sequence typing. Revista Científica do UBM, v. 23, n. 45, p. 129-141, 6 jul. 2021.
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